SECONDARY METABOLITE AND ANTIOXIDANT ACTIVITY OF SOURSOP (ANNONA MONTANA) FRUIT EXTRACT

Abstract

Soursop (Annona montana) is one of the plants that can be used as an antioxidant because it contains terpenoid. Antioxidants are responsible for neutralizing the increase of free radicals. The purpose of this research is to determine the secondary metabolite and antioxidant activity using the DPPH free radicals. This research was included as a descriptive research, which was conducted in the Pharmacognosy Laboratory of Academy of Pharmacy Putra Indonesia Malang. The research stages include the collection of materials, determination of plant, extraction with the maceration method for 5 days using ethanol 70%, qualitative testing of secondary metabolite compounds, testing of antioxidant activity using 2,2-diphenyl-1-picrylhydrazyl (DPPH). The DPPH solution concentration is 100.9 ppm, while the sample concentrations are 60 ppm, 70 ppm, 80 ppm, 90 ppm, and 100 ppm dissolved with ethanol pa and replicated for 3 times. All the solutions were measured their absorbance using the UV-Vis spectrophotometer at 515 nm of wavelength. The finding shows that the secondary metabolite of terpenoid was found on the soursop fruit extract. Also, the IC₅₀ value of 61 ppm was obtained. It can be concluded that the soursop fruit sample of 61 ppm can reduce the free radical level by 50% so it is categorized as a strong antioxidant.